PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of genitmedLink to Publisher's site
 
Genitourin Med. 1987 August; 63(4): 250–255.
PMCID: PMC1194078

Indirect haemagglutination test using gonococcal pilus antigen: how useful to diagnose gonorrhoea?

Abstract

In 1979 an indirect haemagglutination test (gonococcal antibody test) using gonococcal pilus antigen replaced the gonococcal complement fixation test as our routine procedure to show gonococcal antibodies. In the diagnosis of current gonorrhoea the sensitivity of the gonococcal antibody test was far superior to that of the gonococcal complement fixation test (about 55% versus 9% for first episode gonorrhoea). To evaluate the usefulness of the test result the following population groups were studied: 1376 patients undergoing medical examination for gonorrhoea (386 had gonorrhoea), 1384 healthy people aged 15-65, 54 patients with meningococcal disease, 30 children with respiratory tract infection, and 254 patients with evidence of various diseases other than neisserial infections that might be associated with symptoms of arthritis. These investigations showed that (1) non-specific positive gonococcal antibody test results occur rarely, (2) at least half the people who have had gonorrhoea remain seropositive (with titres of 1/40 to 1/160), and (3) a positive test result is more significant the younger the patient and the higher the titre. For younger people a positive test result should always be followed up by bacteriological examination; in all age groups titres of 1/320 or more should indicate medical examination for current gonorrhoea.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (788K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bro-Jorgensen A, Jensen T. Gonococcal pharyngeal infections. Report of 110 cases. Br J Vener Dis. 1973 Dec;49(6):491–499. [PMC free article] [PubMed]
  • Kristensen JK. Genital og ekstragenital gonoré. Et 1 års materiale. Ugeskr Laeger. 1974 Apr 1;136(14):753–759. [PubMed]
  • Buchanan TM, Swanson J, Holmes KK, Kraus SJ, Gotschlich EC. Quantitative determination of antibody to gonococcal pili. Changes in antibody levels with gonococcal infection. J Clin Invest. 1973 Nov;52(11):2896–2909. [PMC free article] [PubMed]
  • Reimann K, Lind I, Andersen KE. An indirect haemagglutination test for demonstration of gonococcal antibodies using gonococcal pili as antigen. II. Serological investigation of patients attending a dermato-venereological outpatients clinic in Copenhagen. Acta Pathol Microbiol Scand C. 1980 Jun;88(3):155–162. [PubMed]
  • Hermodson MA, Chen KC, Buchanan TM. Neisseria pili proteins: amino-terminal amino acid sequences and identification of an unusual amino acid. Biochemistry. 1978 Feb 7;17(3):442–445. [PubMed]
  • REYN A. LABORATORY DIAGNOSIS OF GONOCOCCAL INFECTIONS. Bull World Health Organ. 1965;32:449–469. [PubMed]
  • Vecchio TJ. Predictive value of a single diagnostic test in unselected populations. N Engl J Med. 1966 May 26;274(21):1171–1173. [PubMed]
  • Oates SA, Falkler WA, Jr, Joseph JM, Warfel LE. Asymptomatic females: detection of antibody activity to gonococcal pili antigen by radioimmunoassay. J Clin Microbiol. 1977 Jan;5(1):26–30. [PMC free article] [PubMed]
  • Young H, Low AC. Serological diagnosis of gonorrhoea: detection of antibodies to gonococcal pili by enzyme-linked immunosorbent assay. Med Lab Sci. 1981 Jan;38(1):41–47. [PubMed]
  • Koransky JR, Jacobs NF., Jr Serologic testing for gonorrhea. Sex Transm Dis. 1977 Jan-Mar;4(1):27–31. [PubMed]
  • Dans PE, Rothenberg R, Holmes KK. Gonococcal serology: how soon, how useful, and how much. J Infect Dis. 1977 Feb;135(2):330–334. [PubMed]
  • Gaafar HA. Serologic diagnosis of gonococcal infection. Arch Androl. 1979 Dec;3(4):337–341. [PubMed]
  • Beebe JL. Physician utilization of a gonococcal antibody screening test. Sex Transm Dis. 1983 Oct-Dec;10(4):195–197. [PubMed]

Articles from Genitourinary Medicine are provided here courtesy of BMJ Publishing Group