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J Clin Pathol. 1979 August; 32(8): 814–821.
PMCID: PMC1145815

Platelet function in hairy-cell leukaemia


A quantitative study of various aspects of platelet function was carried out in eight patients with typical hairy-cell leukaemia (HCL). In at least two patients platelet aggregation was convincingly reduced to more than one aggregating agent (ADP, adrenaline, collagen, thrombin, and ristocetin). Granular storage capacity for {14C} 5-HT was reduced in five of the six patients tested. The two patients with definitely abnormal aggregation had the greatest reduction in granular storage pool and the longest bleeding times of those tested but, like the other patients, they did not have a clinical haemostatic defect. It was concluded that a granular storage pool defect (SPD) was at least partly responsible for aggregation abnormalities in HCL since the platelet release reaction in response to thrombin appeared to be normal. All our patients ran a chronic course uncomplicated by any of the factors known to predispose to a platelet SPD acquired in the circulation. Although in the one patient tested before and after splenectomy there was some improvement in platelet aggregation after operation, there was no clear general relationship between defective platelet function and either previous splenectomy or platelet count. Since a direct involvement of the megakaryocytic series in the underlying cell proliferation of HCL seems unlikely, it is concluded that the platelet defect can most reasonably be attributed to the production of abnormal platelets as a result of marrow fibrosis and/or infiltration by hairy cells.

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Selected References

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  • ASTER RH, JANDL JH. PLATELET SEQUESTRATION IN MAN. I. METHODS. J Clin Invest. 1964 May;43:843–855. [PMC free article] [PubMed]
  • Boullin DJ, O'Brien RA. Abnormalities of 5-hydroxytryptamine uptake and binding by blood platelets from children with Down's syndrome. J Physiol. 1971 Jan;212(2):287–297. [PubMed]
  • BOURONCLE BA, WISEMAN BK, DOAN CA. Leukemic reticuloendotheliosis. Blood. 1958 Jul;13(7):609–630. [PubMed]
  • BRECHER G, CRONKITE EP. Morphology and enumeration of human blood platelets. J Appl Physiol. 1950 Dec;3(6):365–377. [PubMed]
  • Catovsky D. Hairy-cell leukaemia and prolymphocytic leukaemia. Clin Haematol. 1977 Feb;6(1):245–268. [PubMed]
  • Costa JL, Murphy DL, Kafka MS. Demonstration and evaluation of apparent cytoplasmic and vesicular serotonin compartments in human platelets. Biochem Pharmacol. 1977 Mar 15;26(6):517–521. [PubMed]
  • Cowan DH, Graham RC, Jr, Baunach D. The platelet defect in leukemia. Platelet ultrastructure, adenine nucleotide metabolism, and the release reaction. J Clin Invest. 1975 Jul;56(1):188–200. [PMC free article] [PubMed]
  • Da Prada M, Pletscher A. Isolated 5-hydroxytryptamine organelles of rabbit blood platelets: physiological properties and drug-induced changes. Br J Pharmacol. 1968 Nov;34(3):591–597. [PubMed]
  • Feiner AS, Meyers AM, Jr, Moore GE. Leukemic reticuloendotheliosis. Loss of platelet defect after splenectomy. JAMA. 1978 Jul 21;240(3):238–240. [PubMed]
  • Gerrard JM, Stoddard SF, Shapiro RS, Coccia PF, Ramsay NK, Nesbit ME, Rao GH, Krivit W, White JG. Platelet storage pool deficiency and prostaglandin synthesis in chronic granulocytic leukaemia. Br J Haematol. 1978 Dec;40(4):597–607. [PubMed]
  • Golde DW, Stevens RH, Quan SG, Saxon A. Immunoglobulin synthesis in hairy cell leukaemia. Br J Haematol. 1977 Mar;35(3):359–365. [PubMed]
  • Golomb HM. Hairy cell leukemia: an unusual lymphoproliferative disease: a study of 24 patients. Cancer. 1978 Aug;42(2 Suppl):946–956. [PubMed]
  • Golomb HM, Vardiman J, Sweet DL, Jr, Simon D, Variakojis D. Hairy cell leukaemia: evidence for the existence of a spectrum of functional characteristics. Br J Haematol. 1978 Feb;38(2):161–170. [PubMed]
  • Hardisty RM, Mills DC, Ketsa-Ard K. The platelet defect associated with albumism. Br J Haematol. 1972 Dec;23(6):679–692. [PubMed]
  • HARDISTY RM, STACEY RS. Platelet 5-hydroxytryptamine (HT) in disorders of the blood. Br J Haematol. 1957 Jul;3(3):292–298. [PubMed]
  • Harker LA, Slichter SJ. Platelet and fibrinogen consumption in man. N Engl J Med. 1972 Nov 16;287(20):999–1005. [PubMed]
  • Hess HH, Lees MB, Derr JE. A linear Lowry--Folin assay for both water-soluble and sodium dodecyl sulfate-solubilized proteins. Anal Biochem. 1978 Mar;85(1):295–300. [PubMed]
  • Jansen J, Hermans J, Remme J, den Ottolander GJ, Cardozo PL. Hairy cell leukaemia. Clinical features and effect of splenectomy. Scand J Haematol. 1978 Jul;21(1):60–71. [PubMed]
  • Katayama I, Finkel HE. Leukemic reticuloendotheliosis. A clinicopathologic study with review of the literature. Am J Med. 1974 Jul;57(1):115–126. [PubMed]
  • Levine PH, Katayama I. The platelet in leukemic reticuloendotheliosis. Functional and morphological evidence of a qualitative disorder. Cancer. 1975 Oct;36(4):1353–1358. [PubMed]
  • Lewis SM, Catovsky D, Hows JM, Ardalan B. Splenic red cell pooling in hairy cell leukaemia. Br J Haematol. 1977 Mar;35(3):351–357. [PubMed]
  • Mielke CH, Jr, Kaneshiro MM, Maher IA, Weiner JM, Rapaport SI. The standardized normal Ivy bleeding time and its prolongation by aspirin. Blood. 1969 Aug;34(2):204–215. [PubMed]
  • Pareti FI, Capitanio A, Mannucci PM. Acquired storage pool disease in platelets during disseminated intravascular coagulation. Blood. 1976 Oct;48(4):511–515. [PubMed]
  • Pletscher A, Da Prada M, Tranzer JP. Metabolism, subcellular transport and storage of 5-hydroxytryptamine in blood platelets. Ann Med Exp Biol Fenn. 1968;46(3):399–406. [PubMed]
  • Reimers HJ, Allen DJ, Cazenave JP, Feuerstein IA, Mustard JF. Serotonin transport and storage in rabbit blood platelets--the effects of reserpine and imipramine. Biochem Pharmacol. 1977 Sep 15;26(18):1645–1655. [PubMed]
  • Skoza L, Zucker MB, Jerushalmy Z, Grant R. Kinetic studies of platelet aggregation induced by adenosine diphosphate and its inhibition by chelating agents, guanidino compounds, and adenosine. Thromb Diath Haemorrh. 1967 Dec 31;18(3-4):713–725. [PubMed]
  • Tuomisto J. A new modification for studying 5-HT uptake by blood platelets: a re-evaluation of tricyclic antidepressants as uptake inhibitors. J Pharm Pharmacol. 1974 Feb;26(2):92–100. [PubMed]
  • Weiss HJ, Tschopp TB, Rogers J, Brand H. Studies of platelet 5-hydroxytryptamine (serotonin) in storage pool disease and albinism. J Clin Invest. 1974 Aug;54(2):421–433. [PMC free article] [PubMed]
  • Zahavi J, Marder VJ. Acquired "storage pool disease" of platelets associated with circulating antiplatelet antibodies. Am J Med. 1974 Jun;56(6):883–890. [PubMed]

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