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J Clin Pathol. 1979 February; 32(2): 187–190.
PMCID: PMC1145607

A new technique for Gram staining paraffin-embedded tissue.

Abstract

Five techniques for Gram staining bacteria in paraffin sections were compared on serial sections of pulmonary tissues from eight bacteriological necropsies. Brown and Hopp's method was the most satisfactory for distinguishing Gram-positive and Gram-negative bacteria. However, this method cannot be recommended as the preparations were frequently overstained, and the Gram-negative bacteria were stained indistinctly. A modification of Brown and Hopps' method was developed which stains larger numbers of Gram-negative bacteria and differentiates well between different cell types and connective tissue, and there is no risk of overstaining.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Adams E. Studies in gram staining. Stain Technol. 1975 Jul;50(4):227–231. [PubMed]
  • Brown RC, Hopps HC. Staining of bacteria in tissue sections: a reliable gram stain method. Am J Clin Pathol. 1973 Aug;60(2):234–240. [PubMed]
  • HUMBERSTONE FD. A GRAM-TYPE STAIN FOR THE SIMULTANEOUS DEMONSTRATION OF GRAM-POSITIVE AND GRAM-NEGATIVE MICRO-ORGANISMS IN PARAFFIN SECTIONS. J Med Lab Technol. 1963 Jul;20:153–156. [PubMed]
  • Taylor RD. Modification of the Brown and Brenn gram stain for the differential staining of gram-positive and gram-negative bacteria in tissue sections. Am J Clin Pathol. 1966 Oct;46(4):472–474. [PubMed]

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