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Logo of jcinvestThe Journal of Clinical Investigation
J Clin Invest. 1983 September; 72(3): 987–996.
PMCID: PMC1129265

Transplacental immunization of the human fetus to tetanus by immunization of the mother.


Experimental studies in rats showed that immunization of the pregnant female led to the transplacental immunization of her fetuses. The possibility that this also occurred in humans was explored by immunizing 42 pregnant women with tetanus toxoid (2.5 or 5 Lf) in the fifth and eighth months of pregnancy and comparing the immune responses of their offspring with the responses of the offspring of 25 unimmunized mothers. Only the offspring of the immunized mothers were sensitized to tetanus. IgM antitetanus antibodies were in their blood before immunization with diphtheria, pertussis, tetanus vaccine (DPT), they had a more rapid (P less than 0.01) response to DPT immunization, and they were still highly sensitized (P less than 0.01) to tetanus 13 mo after birth. In addition, pregnancy had no immunosuppressive effect (P less than 0.05) on the responses of the mothers to tetanus toxoid. Thus, transplacental immunization occurs in humans; it enhances the response of the offspring to subsequent immunization, and it could be used to circumvent the necessity for immunization in early neonatal life.

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Selected References

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  • Gill TJ, 3rd, Kunz HW. Enhanced antibody response in the offspring of immunized rats. J Immunol. 1971 Jan;106(1):274–275. [PubMed]
  • Gill TJ, 3rd, Kunz HW, Bernard CF. Maternal-fetal interaction and immunological memory. Science. 1971 Jun 25;172(3990):1346–1348. [PubMed]
  • Gill TJ, 3rd, Kunz HW, Davis BK. Effect of maternal immunization on the antibody response of low responder rats. Int Arch Allergy Appl Immunol. 1977;53(4):366–375. [PubMed]
  • Davis BK, Gill TJ., 3rd Decreased antibody response in the offspring of immunized high responder rats. J Immunol. 1975 Oct;115(4):1166–1168. [PubMed]
  • Cramer DV, Gill TJ, 3rd, Knauer G. The influence of maternal immunization on the antibody response of the offspring in genetically high-responding rats. Am J Pathol. 1978 Feb;90(2):317–324. [PubMed]
  • IPSEN J., Jr Immunization of adults against diphtheria and tetanus. N Engl J Med. 1954 Sep 16;251(12):459–466. [PubMed]
  • LaForce FM, Young LS, Bennett JV. Tetanus in the United States (1965-1966):epidemiologic and clinical features. N Engl J Med. 1969 Mar 13;280(11):569–574. [PubMed]
  • Newell KW, Leblanc DR, Edsall G, Levine L, Christensen H, Montouri MH, Ramirez N. The serological assessment of a tetanus toxoid field trial. Bull World Health Organ. 1971;45(6):773–785. [PubMed]
  • Relyveld EH, Labusquière R, Gateff C, Le Bourthe F, Ravisse P, Lemarinier G, Chambon L. Antitetanus vaccination and neonatal protection in developing countries. Prog Immunobiol Stand. 1971;5:517–527. [PubMed]
  • Stanfield JP, Gall D, Bracken PM. Single-dose antenatal tetanus immunisation. Lancet. 1973 Feb 3;1(7797):215–219. [PubMed]
  • Garnier MJ, Marshall FN, DAvison KJ, Lepreau FJ., Jr Tetanus in Haiti. Lancet. 1975 Feb 15;1(7903):383–386. [PubMed]
  • SCHOFIELD FD, TUCKER VM, WESTBROOK GR. Neonatal tetanus in New Guinea. Effect of active immunization in pregnancy. Br Med J. 1961 Sep 23;2(5255):785–789. [PMC free article] [PubMed]
  • Gill TJ, 3rd, Repetti CF. Immunologic and genetic factors influencing reproduction. A review. Am J Pathol. 1979 May;95(2):465–570. [PubMed]
  • Repetti CF, Gill TJ., 3rd Radioimmunoassay of IgM and IgG antitetanus toxoid antibody. J Immunol Methods. 1980;37(2):153–163. [PubMed]
  • Gill TJ, 3rd, Rabin BS, Harina BM, Taylor FH. Relationship between the responsiveness of maternal and foetal lymphocytes to phytohaemagglutinin and to microbial antigens. J Immunogenet. 1979 Jun;6(3):197–214. [PubMed]
  • Harina BM, Gill TJ, 3rd, Rabin BS, Taylor FH. A statistical analysis of human lymphocyte transformation data. J Immunogenet. 1979 Jun;6(3):185–196. [PubMed]
  • Evans MI, Schulman JD, Golden L, Mukherjee AB. Superovulation-induced intrauterine growth retardation in mice. Am J Obstet Gynecol. 1981 Oct 15;141(4):433–435. [PubMed]
  • Melsom R, Duncan ME, Harboe M, Bjune G. Antibodies against Mycobacterium leprae antigen 7 from birth to 18 months of age: an indicator of intra-uterine infection in leprosy. Clin Exp Immunol. 1980 Oct;42(1):107–113. [PubMed]
  • Melsom R, Duncan ME, Bjune G. Immunoglobulin concentration in mothers with leprosy and in healthy controls and their babies at the time of birth. Lepr Rev. 1980 Mar;51(1):19–28. [PubMed]
  • Ruben FL, Winkelstein A, Sabbagha RE. In utero sensitization with influenza virus in man. Proc Soc Exp Biol Med. 1975 Sep;149(4):881–883. [PubMed]
  • Cradock-Watson JE, Ridehalgh MK, Anderson MJ, Pattison JR, Kangro HO. Fetal infection resulting from maternal rubella after the first trimester of pregnancy. J Hyg (Lond) 1980 Dec;85(3):381–391. [PMC free article] [PubMed]
  • Papaevangelou G, Hoofnagle J, Kremastinou J. Transplacental transmission of hepatitis-B virus by symptom-free chronic carrier mothers. Lancet. 1974 Sep 28;2(7883):746–748. [PubMed]
  • Aase JM, Noren GR, Reddy DV, Geme JW., Jr Mumps-virus infection in pregnant women and the immunologic response of their offspring. N Engl J Med. 1972 Jun 29;286(26):1379–1382. [PubMed]
  • Brody JI, Oski FA, Wallach EE. Neonatal lymphocyte reactivity as an indicator of intrauterine bacterial contact. Lancet. 1968 Jun 29;1(7557):1396–1398. [PubMed]
  • Bellanti JA, Jackson AL. Characterization of the serum immunoglobulins to the somatic antigen of S. typhosa in an infant following intrauterine immunization. J Pediatr. 1967 Dec;71(6):783–789. [PubMed]
  • Pollack MS, Kirkpatrick D, Kapoor N, Dupont B, O'Reilly RJ. Identification by HLA typing of intrauterine-derived maternal T cells in four patients with severe combined immunodeficiency. N Engl J Med. 1982 Sep 9;307(11):662–666. [PubMed]
  • Gill TJ., 3rd Chimerism in humans. Transplant Proc. 1977 Jun;9(2):1423–1431. [PubMed]
  • Beer AE, Billingham RE. Maternally acquired runt disease. Science. 1973 Jan 19;179(4070):240–243. [PubMed]
  • Hurtenbach U, Sachs DH, Shearer GM. Protection against graft vs. host-associated immunosuppression in F1 mice. I. Activation of F1 regulatory cells by host-specific anti-major histocompatibility complex antibodies. J Exp Med. 1981 Dec 1;154(6):1922–1934. [PMC free article] [PubMed]
  • Naiman JL, Punnett HH, Lischner HW, Destiné ML, Arey JB. Possible graft-versus-host reaction after intrauterine transfusion for Rh erythroblastosis fetalis. N Engl J Med. 1969 Sep 25;281(13):697–701. [PubMed]
  • Parkman R, Mosier D, Umansky I, Cochran W, Carpenter CB, Rosen FS. Graft-versus-host disease after intrauterine and exchange transfusions for hemolytic disease of the newborn. N Engl J Med. 1974 Feb 14;290(7):359–363. [PubMed]
  • Grogan TM, Broughton DD, Doyle WF. Graft-versus-host reaction (GVHR). A case report suggesting GVHR occurred as a result of maternofetal cell transfer. Arch Pathol. 1975 Jun;99(6):330–334. [PubMed]
  • Sasazuki T, Kohno Y, Iwamoto I, Tanimura M, Naito S. Association between an HLA haplotype and low responsiveness to tetanus toxoid in man. Nature. 1978 Mar 23;272(5651):359–361. [PubMed]
  • Schanfield MS, Wells JV, Fudenberg HH. Immunoglobulin allotypes and response to tetanus toxoid in Papua, New Guinea. J Immunogenet. 1979 Oct;6(5):311–315. [PubMed]
  • Sumaya CV, Gibbs RS. Immunization of pregnant women with influenza A/New Jersey/76 virus vaccine: reactogenicity and immunogenicity in mother and infant. J Infect Dis. 1979 Aug;140(2):141–146. [PubMed]
  • Murray DL, Imagawa DT, Okada DM, St Geme JW., Jr Antibody response to monovalent A/New Jersey/8/76 influenza vaccine in pregnant women. J Clin Microbiol. 1979 Aug;10(2):184–187. [PMC free article] [PubMed]
  • Kerman R, Segre D. Anti-pneumococcal polysaccharide type 3 hemolytic plaques in mice: cellular response in immunity and immunologic paralysis. J Immunol. 1970 May;104(5):1262–1266. [PubMed]

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