Purification and Characterization of EDTA Monooxygenase from the EDTA-Degrading Bacterium BNC1

J Bacteriol. 1998 August; 180(15): 3823–3827.

PMCID: PMC107365

Purification and Characterization of EDTA Monooxygenase from the EDTA-Degrading Bacterium BNC1

Jason W. Payne,^1,² Harvey Bolton, Jr.,² James A. Campbell,³ and Luying Xun^1,²^*

Department of Microbiology, Washington State University, Pullman, Washington 99164-4233,¹ and Environmental Microbiology Group² and Advanced Organic Analytical Methods Group,³ Pacific Northwest National Laboratory, Richland, Washington 99352

^*Corresponding author. Mailing address: Department of Microbiology, Washington State University, Pullman, WA 99163-4233. Phone: (509) 335-2787. Fax: (509) 335-1907. E-mail: xun/at/mail.wsu.edu.

Received December 23, 1997; Accepted May 26, 1998.

This article has been corrected. See J Bacteriol. 1998 November; 180(21): 5808.

This article has been cited by other articles in PMC.

Abstract

The synthetic chelating agent EDTA can mobilize radionuclides and heavy metals in the environment. Biodegradation of EDTA should reduce this mobilization. Although several bacteria have been reported to mineralize EDTA, little is known about the biochemistry of EDTA degradation. Understanding the biochemistry will facilitate the removal of EDTA from the environment. EDTA-degrading activities were detected in cell extracts of bacterium BNC1 when flavin mononucleotide (FMN), NADH, and O₂ were present. The degradative enzyme system was separated into two different enzymes, EDTA monooxygenase and an FMN reductase. EDTA monooxygenase oxidized EDTA to glyoxylate and ethylenediaminetriacetate (ED3A), with the coconsumption of FMNH₂ and O₂. The FMN reductase provided EDTA monooxygenase with FMNH₂ by reducing FMN with NADH. The FMN reductase was successfully substituted in the assay mixture by other FMN reductases. EDTA monooxygenase was purified to greater than 95% homogeneity and had a single polypeptide with a molecular weight of 45,000. The enzyme oxidized both EDTA complexed with various metal ions and uncomplexed EDTA. The optimal conditions for activity were pH 7.8 and 35°C. K_ms were 34.1 μM for uncomplexed EDTA and 8.5 μM for MgEDTA²⁻; this difference in K_m indicates that the enzyme has greater affinity for MgEDTA²⁻. The enzyme also catalyzed the release of glyoxylate from nitrilotriacetate and diethylenetriaminepentaacetate. EDTA monooxygenase belongs to a small group of FMNH₂-utilizing monooxygenases that attack carbon-nitrogen, carbon-sulfur, and carbon-carbon double bonds.

Synthetic chelating agents have a wide variety of uses, from household cleaners, water treatment, and pulp and paper bleaching to rubber and metal processing (2, 23, 30). About 70% of the chelating agents used worldwide are aminopolycarboxylic acids, primarily EDTA, diethylenetriaminepentaacetate (DTPA), and nitrilotriacetate (NTA), with annual production about 372 million lb in the United States, western Europe, and Japan in 1994 (30). EDTA is the most commonly used chelating agent. In the environment, EDTA has some undesirable environmental consequences such as the remobilization of radionuclides and heavy metals from soils and sediments (6, 9, 24). The mobilized radionuclides and toxic heavy metals can cause direct health problems or can be accumulated by plants and then transferred to human beings through the food chain. EDTA is recalcitrant in soils (25, 34, 35) and sediments (3, 34, 35). Photodegradation of FeEDTA plays a major role in the fate of EDTA in surface waters (13, 14). Other species, such as CuEDTA, ZnEDTA, and NiEDTA, are not degraded by sunlight, whereas CoEDTA is only slightly sensitive (20, 26).

To date, only two microorganisms have been reported to degrade EDTA. The gram-negative bacterium BNC1 was isolated from sewage receiving EDTA-containing wastewater effluents (27); an Agrobacterium sp. growing on Fe(III)EDTA has also been isolated (18). However, little is known about the biochemistry of EDTA degradation by these microorganisms. To facilitate bioremediation of EDTA and reduce the mobility of heavy metals in the groundwater, the biochemistry of EDTA degradation by bacterium BNC1 was studied. We report here the purification and characterization of EDTA monooxygenase (EDTA-Mo), which catalyzed the first step of EDTA degradation by bacterium BNC1.

(A preliminary account of this work was presented at the 96th General Meeting of the American Society for Microbiology [28].)

MATERIALS AND METHODS

Bacterium and culture conditions.

The EDTA-degrading bacterium BNC1 was kindly provided by Bernd Nörtemann (Technical University of Braunschweig, Braunschweig, Germany). BNC1 was cultured with Na₂EDTA · 2H₂O (0.3 g/liter) and potassium acetate (0.25 g/liter) in a mineral medium (27). Large quantities of cells were obtained by culturing BNC1 in a 50-liter carboy containing 30 liters of the medium bubbled with sterile air for 2.5 days at 24°C. Toward the end of log phase, cells were harvested by concentrating the culture to 2 liters in a hollow fiber filtration unit (model DC10L system; Amicon, Beverly, Mass.) and then centrifuged at 17,000 × g for 15 min at 4°C. The cells were stored at −20°C for a maximum of 3 days.

Enzyme assays.

EDTA-Mo activity was assayed by measuring the production of glyoxylate. A standard assay mixture contained 20 mM HEPES buffer (pH 7.8), 10 μM flavin mononucleotide (FMN), 2 mM NADH, 500 μM Na₂EDTA (or other chelator), 500 μM MgCl₂, 0.2 U of NAD(P)H:FMN oxidoreductase from Photobacterium fischeri (Boehringer Mannheim Co., Indianapolis, Ind.), 30 U of catalase (from bovine liver; Sigma), and an appropriate amount of EDTA-Mo preparation in a total volume of 250 μl. The NAD(P)H:FMN oxidoreductase generated FMNH₂ by reducing FMN with NADH and was added in excess to ensure sufficient amounts of FMNH₂ for EDTA-Mo. The reaction was initiated by adding NADH. A stock solution of 100 mM NADH was prepared in 10 mM Tris base (pH >13). The assay was stopped by adding 100 μl of 0.1 N HCl. The glyoxylate produced was detected by phenylhydrazine-K₃Fe(CN)₆ as previously described (37). The aqueous speciation of EDTA among its free acid and complexed forms (e.g., H₂EDTA²⁻ and MgEDTA²⁻) was calculated with an aqueous speciation-solubility model MINTEQA2 (1, 31) as previously described (39).

Purification steps.

All operations were performed at 6°C. All buffers except those used for the hydroxyapatite column contained 1 mM dithiothreitol. The levels of ammonium sulfate saturation were those at 25°C.

(i) Extraction of cells.

About 15 g of frozen cells was thawed at room temperature and suspended in 30 ml of 20 mM Tris buffer (pH 8.0) containing 2.5 mM EDTA. The protease inhibitor phenylmethylsulfonyl fluoride was freshly prepared in absolute ethanol at a concentration of 30 mM and added to the cell suspension to a final concentration of 0.5 mM. The slurry was passed through a French pressure cell (model FA-030; Aminco, Urbana, Ill.) three times at 260 MPa. The product was centrifuged at 17,000 × g for 15 min. The supernatant was saved, and the pellet was discarded.

(ii) Protamine sulfate treatment.

A 2% solution of protamine sulfate in 20 mM Tris buffer (pH 8.0) was added to the supernatant slowly to 0.05% with stirring. After 5 min, the mixture was centrifuged at 17,000 × g for 15 min, and the supernatant was saved.

(iii) Ammonium sulfate fractionation.

Solid ammonium sulfate was added to the supernatant to 33% saturation with constant stirring. The pH of the solution was not adjusted. After 10 min of stirring, the mixture was centrifuged at 17,000 × g for 15 min. The precipitate was discarded. Additional solid ammonium sulfate was added to the supernatant to 70% saturation with constant stirring. After 10 min of stirring, the mixture was centrifuged at 17,000 × g for 15 min. The precipitate was saved, and the supernatant was discarded.

(iv) Ultracentrifugation.

The precipitate was suspended in an equal volume of 25 mM potassium phosphate (KPi) buffer (pH 7.0). The suspension was dialyzed against 1 liter of the same buffer for 2.5 h. The dialyzed sample was centrifuged at 331,000 × g for 40 min, and the supernatant was saved.

(v) Dye chromatography.

The supernatant was loaded onto a 25-ml Cibacron Blue 3GA agarose (Sigma) column (1.5 by 14 cm) previously equilibrated with 25 mM KPi buffer (pH 7.0). A maximum of 150 mg of total protein was loaded per run. After the proteins were loaded, the column was washed with 80 ml of the equilibrating buffer and then washed with 80 ml of 1 M NaCl in 25 mM KPi buffer (pH 7.0). Most proteins did not bind to the column and washed off with the starting buffer. Both EDTA-Mo and an FMN reductase were eluted with the 1 M NaCl solution. The eluent containing the enzymes was concentrated to 1 ml with a Centriprep-10 (Millipore). The sample was desalted to 25 mM KPi (pH 7.0) with a Centriprep-10.

(vi) MonoQ chromatography.

The sample from the dye column in 25 mM KPi (pH 7.0) was injected onto a MonoQ HR 5/5 column (Pharmacia) equilibrated with the same buffer. Proteins were eluted with a step and linear gradient of NaCl (percentages of 1 M NaCl in the same buffer: 0%, 4 ml; 0 to 15%, 20-ml linear gradient; 100%, 6 ml; and 0%, 3 ml) by a fast protein liquid chromatography (FPLC) system (Pharmacia). EDTA-Mo was eluted associated with a major peak around 80 mM NaCl. The fractions containing the enzyme activity were pooled and concentrated to less than 1 ml with a Centriprep-10. The FMN reductase was not bound to the column and was separated from EDTA-Mo by this step.

(vii) Hydroxyapatite chromatography.

The buffer for EDTA-Mo was changed to 8 mM KPi (pH 6.6) with a Centriprep-10. The sample was injected onto a Bio-Scale CHT2-I hydroxyapatite column (Bio-Rad, Hercules, Calif.) equilibrated with the same buffer. Proteins were eluted with a step and linear gradient of KPi (pH 6.6) (concentrations of KPi: 8 mM, 4 ml; 50 mM, 5 ml; 50 to 250 mM, 20-ml linear gradient; and 500 mM, 8 ml) by FPLC. EDTA-Mo was eluted as a major peak around 165 mM KPi, concentrated to 1 ml with a Centriprep-10, and stored at −80°C.

Analytical methods.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was done by the method of Laemmli (17). Gels were stained for proteins with Coomassie brilliant blue R-250. Protein concentrations were determined by a protein dye reagent (4), with bovine serum albumin as the standard. Gel filtration chromatography was used to estimate the native molecular weight of the enzyme. Purified enzyme was injected onto a Superose 12 column (Pharmacia) equilibrated with 25 mM KPi (pH 7.0) containing 150 mM NaCl. The enzyme was eluted with the same buffer by FPLC. The EDTA-Mo was eluted off the column as a single peak with a retention volume of 12.5 ml. Gel filtration standards were purchased from Bio-Rad. The nitrogen-containing moiety of enzymatic end product from NTA was derivatized by 9-fluorenylmethylchloroformate (7) and then determined by a high-performance liquid chromatography (HPLC) system (Waters). The N-terminal amino acid sequence of the purified protein was determined on an ABI 470 protein sequencer at the Department of Biochemistry and Biophysics, Washington State University, as previously described (38).

Oxygen consumption.

Oxygen consumption by FMNH₂ and by EDTA-Mo was determined in a closed reaction vessel (0.6-ml total volume) fitted with a Clarke-type oxygen electrode (Instech, Plymouth Meeting, Pa.). The electrode was calibrated by N-methylphynazonium methosulfate and NADH (29). The reaction was in 20 mM HEPES buffer (pH 7.8)–10 μM FMN–500 μM Na₂EDTA–500 μM MgCl₂. Two sets of experiments were carried out. The first set contained 0.8 U of NAD(P)H:FMN oxidoreductase only. The second set contained 0.8 U of NAD(P)H:FMN oxidoreductase and 580 μg of EDTA-Mo from the MonoQ column. In both cases, NADH was added in a 2.5-μl volume to a final concentration of 198 μM to initiate O₂ consumption. When O₂ consumption stopped, 90 U of catalase was added to release O₂ from H₂O₂. For the reaction containing EDTA-Mo, the glyoxylate produced was also quantified.

pH and temperature optima.

The EDTA-Mo activities were measured at various pH values within the range of 6.6 to 8.4 with 20 mM HEPES buffer. The enzyme assay was performed as described above except that the reaction was stopped by heating at 90°C for 5 min instead of acidification. The temperature optima for the enzyme activity were determined in a similar way at pH 7.7. The reaction mixture without NADH was incubated at the corresponding temperature for 5 min, and then NADH was added to the mixture to start the reaction. The reaction was terminated by acidification.

Product determination by mass spectroscopy.

A 5-ml aliquot of the reaction mixture was lyophilized with an LPH LOCK (LABCONCO) for approximately 12 h. The dried material was transferred to a Reactivial (VWR Scientific); 2 ml of 12% BF₃–methanol (Aldrich) was added, and the sample was heated to 100°C for 1 h. The solution was cooled, and 2 ml of chloroform was added. The entire solution was then quenched in a vial containing 10 ml of 0.4 M KH₂PO₄ (pH 9.5). The vial was vortexed, and the aqueous and chloroform layers were allowed to separate. The chloroform layer, containing the derivatized organics, was retained, and the aqueous layer was discarded. The chloroform solution was then analyzed by gas chromatography-mass spectrometry (GC-MS) (5, 10).

A model 5890 gas chromatograph (Hewlett-Packard) equipped with a fused silica column (DB-5, 30 m by 0.25 mm [inside diameter], 0.25-μm film thickness; J & W Scientific) was interfaced to a model 5970 mass selective detector (Hewlett-Packard). The oven temperature was programmed in the following manner: 50°C for 1 min, 8°C/min to 260°C, and hold at 260°C for 10 min. The mass selective detector was tuned with perfluorotributylamine. In these studies, the detector was scanned from 50 to 600 and operated in the electron impact mode (70 eV). The source temperature was 200°C, the injector port temperature was 250°C, and the interfaces were at 250°C.

RESULTS

Identification of EDTA degradation in cell extracts.

Cell extracts of BNC1 cultured with EDTA and acetate in a mineral medium were able to degrade EDTA to glyoxylate in the presence of NADH, FMN, and O₂. Flavin adenine dinucleotide (FAD) and riboflavin could not replace FMN. The effects of temperature and pH on glyoxylate production by cell extracts were determined. Optimal reaction rates were achieved at 35°C and pH 7.7. The rates of glyoxylate formation were also affected by the metal cation complexed by EDTA in the assay. Dialyzed cell extracts were able to release glyoxylate from EDTA complexed with any of the cations tested (Mg²⁺, Mn²⁺, Ni²⁺, Co²⁺, Zn²⁺, Fe²⁺, Ca²⁺, Cu²⁺, Cr²⁺, Sn²⁺, Ba²⁺, Cd²⁺, Sr²⁺, Pd²⁺, Al³⁺, Cr³⁺, K⁺, or Na⁺). MgEDTA²⁻ was found to be the best substrate, having a rate nearly twice that of uncomplexed EDTA. For this reason, MgCl₂ was used in the assay.

Enzyme purification.

EDTA-Mo was purified by monitoring glyoxylate production from EDTA in reaction mixtures following each purification step. In the cell extract, there was an FMN reductase that was later separated from EDTA-Mo on a MonoQ column. After the separation, the FMN reductase could be substituted by an NADH:FMN oxidoreductase of Chelatobacter heintzii (38) or an NAD(P)H:FMN oxidoreductase of P. fischeri (36) to generate FMNH₂ for EDTA-Mo. Because its activity can be replaced by other NADH:FMN oxidoreductases, we purified EDTA-Mo with the NAD(P)H:FMN oxidoreductase of P. fischeri to supply FMNH₂. The results of a typical purification of EDTA-Mo are summarized in Table Table1.1.

TABLE 1

Purification scheme for

EDTA-Mo

Enzyme properties and activities.

EDTA-Mo was apparently purified to homogeneity as indicated by SDS-PAGE analysis, revealing a single 45-kDa band (Fig. (Fig.1).1). The native EDTA-Mo was estimated to be a monomer by gel filtration chromatography. The N-terminal sequences of EDTA-Mo was determined to be MNKVLMYL.

FIG. 1

SDS-PAGE of purified EDTA-Mo. Lane 1, low-range molecular weight standards (Bio-Rad); lane 2, 1 μg of EDTA-Mo.

EDTA-Mo produced glyoxylate from EDTA, NTA, and DTPA, with the coconsumption of FMNH₂ and O₂. FMNH₂ was generated by an FMN reductase with NADH as the reductant in the assay system. In the reaction mixture, O₂ consumption was observed even with NAD(P)H:FMN oxidoreductase alone. When 198 μM NADH was added to the reaction mixture in a closed vessel, 225 μM O₂ was consumed in 12 min. The consumed O₂ was quantitatively converted to H₂O₂ since 116 μM O₂ was released when catalase was added. When the reaction mixture also contained EDTA-Mo, 192 μM glyoxylate was produced and 197 μM O₂ was consumed in 4 min. Only 10 μM O₂ was converted to H₂O₂ since only 5 μM O₂ was released when catalase was added.

The end product with the nitrogen moiety from NTA was identified as iminodiacetate by HPLC analysis. The enzyme did not release glyoxylate from commercial iminodiacetate (Sigma). The end product with the nitrogen moiety from EDTA was not detected by the HPLC method and was analyzed by GC-MS. The total ion chromatogram is shown in Fig. Fig.2A.2A. On the basis of the mass spectrum of the component at the retention time of 24.1 min, it was identified as the methyl ester of the lactam of ethylenediaminetriacetate (ED3A) (Fig. (Fig.2B).2B). ED3A forms an internal amino carboxylic ester bond to yield the lactam of ED3A during derivatization before GC-MS analysis (10). The compound at the retention time of 26.7 min was identified as the methyl ester of EDTA by its mass spectrum (data not shown). Neither unsymmetric nor symmetric ethylendiaminediacetate (EDDA) was detected. The first peak in Fig. Fig.2A2A was caused by HEPES used as a buffer for the enzymatic assay. The nitrogen-containing end product from DTPA was not identified because the enzyme did not produce enough of it for GC-MS analysis.

FIG. 2

GC-MS analysis of the end products of EDTA degradation by EDTA-Mo. (A) Total ion chromatogram of the sample; (B) mass spectrum of lactam ED3A.

The effects of temperature and pH on EDTA-Mo activity were determined. The optimal temperature for EDTA-Mo activity was 35°C, with 75, 93, and 96% of the optimal activity retained at 25, 30, and 40°C, respectively. The optimal pH was 7.7 in 20 mM HEPES buffer; the activities were 75, 83, and 70% of the optimum at pH 6.9, 7.3, and 8.1, respectively. The best activity was obtained in 20 mM HEPES buffer (pH 7.7) at 35°C.

Kinetic analysis.

The kinetic parameters were determined for EDTA-Mo with an excess of NAD(P)H:FMN oxidoreductase of P. fischeri to generate FMNH₂. K_m and V_max values were determined for EDTA-Mo from Lineweaver-Burke plots of initial reaction rates. The degradation of EDTA or MgEDTA²⁻ was measured based on the formation of glyoxylate. In the assay mixture without Mg²⁺, EDTA did not associate with Na⁺ and was present as uncomplexed EDTA in the forms of 94% HEDTA³⁻ and 6% H2EDTA²⁻. For uncomplexed EDTA, K_m was 34.1 μM, V_max was 3.7 μM mg⁻¹ min⁻¹, k_cat was 2.8 s⁻¹, and k_cat/K_m was 0.08 μM⁻¹ s⁻¹. When equimolar Mg²⁺ and EDTA were added to the reaction mixture, 98.8% of the EDTA was present as MgEDTA²⁻. For MgEDTA²⁻, K_m was 8.5 μM, V_max was 4.7 μM mg⁻¹ min⁻¹, k_cat was 3.6 s⁻¹, and k_cat/K_m was 0.43 μM⁻¹ s⁻¹. The difference in k_cat/K_m for the two substrates (MgEDTA²⁻ 5.3 times higher) was primarily due to the difference in the K_m, indicating that the enzyme has a greater affinity for the chelate when it is complexed with Mg²⁺.

DISCUSSION

This is the first report that EDTA-Mo has been identified, purified, and characterized. When supplied with FMNH₂, the enzyme appears to break the N-C bond in EDTA, NTA, and DTPA, with one oxygen atom appearing in glyoxylate. The enzyme was able to degrade EDTA, NTA, and DTPA with a variety of metal cations. With cell extracts, the highest rate was found when Mg²⁺ was added. The purified enzyme showed similar preferences for MgEDTA²⁻ and free EDTA. Kinetic analyses indicate that the enzyme has a higher affinity for MgEDTA²⁻. This finding coincides with Mg²⁺ being the most abundant intracellular cation in several tested microorganisms (12), with MgEDTA²⁻ the likely form of EDTA in the cytoplasm of BNC1.

HPLC, GC-MS, and colorimetric analyses showed that EDTA was oxidized to glyoxylate and ED3A, and NTA was oxidized to glyoxylate and iminodiacetate by EDTA-Mo (Fig. (Fig.3).3). Upon examination of the structures of NTA and ED3A, we wondered whether EDTA-Mo could also degrade ED3A to EDDA. However, GC-MS analysis did not detect any trace of either symmetric or unsymmetric EDDA. We are currently cloning the gene encoding EDTA-Mo by using a DNA probe generated from the N-terminal amino acid sequence. Successful production of a functional EDTA-Mo in an expression host will allow us to confirm the identities of enzyme end products from EDTA and NTA as well as to accumulate enough end products from DTPA for identification. Characterization of the gene sequence will reveal the relationships among related enzymes, especially between EDTA-Mo and NTA monooxygenase (NTA-Mo).

FIG. 3

Proposed conversion of EDTA to ED3A and glyoxylate by EDTA-Mo.

The stoichiometry of EDTA oxidation by EDTA-Mo was studied. One molecule of FMN was reduced to one FMNH₂ by NAD(P)H:FMN oxidoreductase at the expense of one NADH. Then one FMNH₂ reacted with one O₂ chemically to generate one H₂O₂. When catalase was added, one O₂ was produced from two H₂O₂. When EDTA-Mo was also present in the reaction mixture, the enzyme consumed one O₂ and one FMNH₂ to oxidized EDTA and produced one glyoxylate. It took 12 min to complete O₂ consumption in the absence of EDTA-Mo but only 4 min in the presence of EDTA-Mo. Ninety-five percent of FMNH₂ generated by NAD(P)H:FMN oxidoreductase was used by EDTA-Mo to oxidize EDTA, and only 5% reacted with O₂ to produce H₂O₂. Although the concentrations of EDTA and ED3A were not determined, the conversion of EDTA to ED3A was determined by GC-MS analysis. On the basis of these data, the reaction catalyzed by EDTA-Mo is proposed (Fig. (Fig.33).

When the kinetic parameters for EDTA oxidation were determined, excess NAD(P)H:FMN oxidoreductase was added in the reaction mixture. Under such conditions, a substantial amount of H₂O₂ was produced. Catalase was added to the reaction mixture to prevent the buildup of H₂O₂. This practice provided sufficient amount of FMNH₂ to EDTA-Mo. When O₂ consumption was studied, excess EDTA-Mo was added to effectively utilize FMNH₂ so that the formation of H₂O₂ was minimized. Because of FMNH₂ is highly reactive with O₂, we could not use O₂ as the limiting factor in the reaction mixture and thus could not determine its kinetic parameters.

There are similarities and differences between EDTA-Mo of BNC1 and NTA-Mo (formerly component A) of C. heintzii (37, 38). Both enzymes require FMNH₂ and O₂ as cosubstrates, and both are single polypeptides of about 50 kDa. EDTA-Mo oxidizes EDTA to ED3A and glyoxylate, and oxidizes NTA to iminodiacetate and glyoxylate. NTA-Mo oxidizes NTA to iminodiacetate and glyoxylate (37, 38). NTA-Mo degrades only specific metal-NTA complexes (37, 39), while EDTA-Mo degrades EDTA, NTA, and DTPA in the absence or presence of metal cations. NTA-Mo does not degrade EDTA or DTPA.

Flavin-dependent monooxygenases are ubiquitous. FAD and FMN are normally prosthetic groups, not cosubstrates, and they are reduced by the monooxygenases themselves with NADH or NADPH as the reductant (8, 32). EDTA-Mo uses FMNH₂ directly. Since the enzyme does not appear to contain any chromophores and does not require any specific transitional metal cofactors, the activated oxygen species is likely a C(4a)-flavin hydroperoxide as proposed for other flavin-dependent monooxygenases (15, 22). Thus, FMNH₂ acts as both reductant and prosthetic group for EDTA-Mo. An endogenous FMN reductase supplied EDTA-Mo with FMNH₂. Since the reductase was replaced by two other FMN reductases, it is unlikely that there is any direct protein-protein interactions between the reductase and oxygenase. These data suggest that EDTA-Mo belongs to a small group of monooxygenases that utilize FMNH₂ as both reductant and prosthetic group. Bacterial luciferase of P. fischeri was the first FMNH₂-utilizing monooxygenase studied (36). Recently, pristinamycin IIA synthase of Streptomyces pristinaespiralis (33), NTA-Mo of C. heintzii (38), and two monooxygenases involved in desulfurization of dibenzothiophene by Rhodococcus sp. strain IGTS8 (11, 19) have been characterized and shown to use FMNH₂ as the cosubstrate. EDTA-Mo appears to be the sixth member of this group. These FMNH₂-dependent monooxygenases appear to attack carbon-nitrogen, carbon-sulfur, carbon-carbon, or carbon-oxygen double bonds.

ACKNOWLEDGMENTS

This research was supported by the Microbial Biotechnology Initiative at Pacific Northwest National Laboratory. It was also partially supported by the Subsurface Science Program, Office of Health and Environmental Research, U.S. Department of Energy (DOE). The support of Frank Wobber is greatly appreciated. Pacific Northwest National Laboratory is operated for the DOE by Battelle Memorial Institute under contract DE-AC06-76RLO 1830.

ADDENDUM

During review of the manuscript, Witschel et al. reported the identification and characterization of a similar EDTA-degrading enzyme from a different bacterial isolate (37a).

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