PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of brjvendisLink to Publisher's site
 
Br J Vener Dis. 1984 December; 60(6): 357–363.
PMCID: PMC1046381

Attachment of Treponema pallidum to fibronectin, laminin, collagen IV, and collagen I, and blockage of attachment by immune rabbit IgG.

Abstract

As shown by scanning electron and phase contrast microscopy, Treponema pallidum attached in vitro to basement membranes purified from kidney cortex tissues or from retinal vessels. This organism also attached to the extracellular matrix remaining after cultured cells had been solubilised with Triton X. Fibronectin, laminin, collagen, IV, collagen I, and hyaluronic acid are structural components of basement membranes and extracellular matrices. Experiments were performed to investigate the in vitro attachment of T pallidum to each of these components. Viable or heat inactivated treponemes were added to glass coverslips precoated with different concentrations of each component. After various times of incubation, coverslips were washed and the attached organisms were counted. Large numbers of viable organisms attached to each of these five components. In contrast, heat inactivation sharply reduced numbers of attached organisms. The IgG fractions of immune and non-immune rabbit serum samples were affinity purified using protein A. T pallidum was preincubated with both fractions, then incubated with either intact cultured cells or with coverslips coated with the five tissue components. The IgG from immune serum blocked treponemal attachment to the cultured cells and to fibronectin, laminin, collagen IV, and collagen I, but not to hyaluronic acid. These results are discussed in terms of attachment mechanisms of T pallidum and potential applications to in vivo infection.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.4M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Fitzgerald TJ. Pathogenesis and immunology of Treponema pallidum. Annu Rev Microbiol. 1981;35:29–54. [PubMed]
  • Fitzgerald TJ, Miller JN, Sykes JA. Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival. Infect Immun. 1975 May;11(5):1133–1140. [PMC free article] [PubMed]
  • Hayes NS, Muse KE, Collier AM, Baseman JB. Parasitism by virulent Treponema pallidum of host cell surfaces. Infect Immun. 1977 Jul;17(1):174–186. [PMC free article] [PubMed]
  • Fieldsteel AH, Stout JG, Becker FA. Comparative behavior of virulent strains of Treponema pallidum and Treponema pertenue in gradient cultures of various mammalian cells. Infect Immun. 1979 May;24(2):337–345. [PMC free article] [PubMed]
  • Fitzgerald TJ, Johnson RC, Miller JN, Sykes JA. Characterization of the attachment of Treponema pallidum (Nichols strain) to cultured mammalian cells and the potential relationship of attachment to pathogenicity. Infect Immun. 1977 Nov;18(2):467–478. [PMC free article] [PubMed]
  • Foidart JM, Bere EW, Jr, Yaar M, Rennard SI, Gullino M, Martin GR, Katz SI. Distribution and immunoelectron microscopic localization of laminin, a noncollagenous basement membrane glycoprotein. Lab Invest. 1980 Mar;42(3):336–342. [PubMed]
  • Repesh LA, Furcht LT, Smith D. Immunocytochemical localization of fibronectin in limb tissues of the adult newt, Notophthalmus viridescens. J Histochem Cytochem. 1981 Aug;29(8):937–945. [PubMed]
  • Timpl R, Glanville RW, Wick G, Martin GR. Immunochemical study on basement membrane (type IV) collagens. Immunology. 1979 Sep;38(1):109–116. [PubMed]
  • Culp LA, Rollins BJ, Buniel J, Hitri S. Two functionally distinct pools of glycosaminoglycan in the substrate adhesion site of murine cells. J Cell Biol. 1978 Dec;79(3):788–801. [PMC free article] [PubMed]
  • Lehto VP, Vartio T, Virtanen I. Fibronectin remains in the cytoskeletal preparations of cultured human fibroblasts. Cell Biol Int Rep. 1981 Apr;5(4):417–426. [PubMed]
  • Terranova VP, Liotta LA, Russo RG, Martin GR. Role of laminin in the attachment and metastasis of murine tumor cells. Cancer Res. 1982 Jun;42(6):2265–2269. [PubMed]
  • Yamada KM, Olden K. Fibronectins--adhesive glycoproteins of cell surface and blood. Nature. 1978 Sep 21;275(5677):179–184. [PubMed]
  • Kuusela P. Fibronectin binds to Staphylococcus aureus. Nature. 1978 Dec 14;276(5689):718–720. [PubMed]
  • Beachey EH, Simpson WA. The adherence of group A streptococci to oropharyngeal cells: the lipoteichoic acid adhesin and fibronectin receptor. Infection. 1982;10(2):107–111. [PubMed]
  • Myhre EB, Kuusela P. Binding of human fibronectin to group A, C, and G streptococci. Infect Immun. 1983 Apr;40(1):29–34. [PMC free article] [PubMed]
  • Grinnell F, Feld MK. Initial adhesion of human fibroblasts in serum-free medium: possible role of secreted fibronectin. Cell. 1979 May;17(1):117–129. [PubMed]
  • Klebe RJ. Isolation of a collagen-dependent cell attachment factor. Nature. 1974 Jul 19;250(463):248–251. [PubMed]
  • Murray JC, Liotta L, Rennard SI, Martin GR. Adhesion characteristics of murine metastatic and nonmetastatic tumor cells in vitro. Cancer Res. 1980 Feb;40(2):347–351. [PubMed]
  • SYKES JA, MOORE EB. A simple tissue culture chamber. Tex Rep Biol Med. 1960;18:288–297. [PubMed]
  • Quist EE, Repesh LA, Zeleznikar R, Fitzgerald TJ. Interaction of Treponema pallidum with isolated rabbit capillary tissues. Br J Vener Dis. 1983 Feb;59(1):11–20. [PMC free article] [PubMed]
  • Fitzgerald TJ, Cleveland P, Johnson RC, Miller JN, Sykes JA. Scanning electron microscopy of Treponema pallidum (Nichols strain) attached to cultured mammalian cells. J Bacteriol. 1977 Jun;130(3):1333–1344. [PMC free article] [PubMed]
  • Fitzgerald TJ, Repesh LA, Oakes SG. Morphological destruction of cultured cells by the attachment of Treponema pallidum. Br J Vener Dis. 1982 Feb;58(1):1–11. [PMC free article] [PubMed]
  • Staat RH, Langley SD, Doyle RJ. Streptococcus mutans adherence: presumptive evidence for protein-mediated attachment followed by glucan-dependent cellular accumulation. Infect Immun. 1980 Feb;27(2):675–681. [PMC free article] [PubMed]
  • Wu-Yuan CD, Tai S, Slade HD. Properties of Streptococcus mutans grown in a synthetic medium: binding of glucosyltransferase and in vitro adherence, and binding of dextran/glucan and glycoprotein and agglutination. Infect Immun. 1979 Mar;23(3):600–608. [PMC free article] [PubMed]
  • Eshdat Y, Speth V, Jann K. Participation of pili and cell wall adhesion in the yeast agglutination activity of Escherichia coli. Infect Immun. 1981 Dec;34(3):980–986. [PMC free article] [PubMed]
  • Fitzgerald TJ, Johnson RC, Ritzi DM. Relationship of Treponema pallidum to acidic mucopolysaccharides. Infect Immun. 1979 Apr;24(1):252–260. [PMC free article] [PubMed]
  • Alderete JF, Baseman JB. Surface characterization of virulent Treponema pallidum. Infect Immun. 1980 Dec;30(3):814–823. [PMC free article] [PubMed]
  • Baseman JB, Hayes EC. Molecular characterization of receptor binding proteins and immunogens of virulent Treponema pallidum. J Exp Med. 1980 Mar 1;151(3):573–586. [PMC free article] [PubMed]
  • Fieldsteel AH, Cox DL, Moeckli RA. Cultivation of virulent Treponema pallidum in tissue culture. Infect Immun. 1981 May;32(2):908–915. [PMC free article] [PubMed]
  • Peterson KM, Baseman JB, Alderete JF. Treponema pallidum receptor binding proteins interact with fibronectin. J Exp Med. 1983 Jun 1;157(6):1958–1970. [PMC free article] [PubMed]

Articles from The British Journal of Venereal Diseases are provided here courtesy of BMJ Publishing Group