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A vitrectomy specimen obtained with the roto-extractor from a diabetic patient with vitreous haemorrhage revealed fungal endophthalmitis on microscopical examination. Endophthalmitis was not suspected before the procedure. Extensive medical examinations failed to reveal any evidence of systemic fungal disease. The case exemplifies endogenous mycotic endophthalmitis of occult origin, the first of its kind, we believe, to be diagnosed in this manner.